Histology Solutions

HistoSpring offers precision tissue and slide preparation, immunostaining and pathology analysis, antibody optimization, and custom requests. Our team of experts provide active collaboration and tactical solutions for successful results.

Not all staining is the same.

Our knowledge of chemistry, staining techniques and troubleshooting staining for tissue sections and analysis of multiple biomarkers requires expertise. HistoSpring offers commonly used stains to special /custom staining to improve research and clinical outcomes.
automated histology
large scale processing, automated H&E  

Routine Histology

The path from research through pre-clinical starts with best-practice techniques, skills and sound procedures.

  • Selection of fixation techniques
  • Frozen OCT embedding
  • Fixed tissue processing and paraffin embedding
  • Microtome sectioning 4 – 20 µm
  • Hematoxylin and Eosin (H & E)

Histochemical Specialty Stains

HistoSpring’s team of experts provide staining techniques to enhance tissue contrast for analysis and biomarker localization.

  • Alizarin Red
  • Masson’s Trichrome
  • Naphthol-AS-D-chloroacetate Esterase
  • Oil Red O
  • Periodic Acid-Schiff Stain
  • Picosirius Red
  • Toluidine Blue


HistoSpring offers custom antibody solutions to improve IHC results and to target cellular biomarkers and analysis. Our team of experts trouble shoot and collaborate to achieve optimum experiment results.

  • Antibody optimization and validation
  • Primary and secondary antibody selection and optimization
  • Detection of low-abundance targets
  • Fluorescent imaging
dual immunofluorescence, co-staining, ICC & IHC staining, double staining immunohistochemistry, immunostaining

Optimized Antibodies for IHC Research in Mouse and Human

  • Ki67
  • α smooth muscle actin
  • Vimentin
  • Pan-Keratin
  • CD3
  • F4/80
  • Estrogen receptor α
  • Progesterone Receptor
  • TUNEL assay